Journal: Einstein

Article Title: Regulatory T cells isolated from endometriotic peritoneal fluid express a different number of Toll-like receptors

doi: 10.31744/einstein_journal/2020AO5294

Figure Lengend Snippet: Expression of Toll-like receptors by peritoneal regulatory T-cells from patients with endometriosis and controls

Article Snippet: Foram usados testes TaqMan de expressão gênica (Applied Biosystems) para os genes-alvo TLR1 (Hs00413978_m1), TLR2 (Hs00610101_m1), TLR3 (Hs01551078_m1), TLR4 (Hs00152939_m1), TLR5 (Hs00152825_m1), TLR6 (Hs00271977_s1), TLR7 (Hs00152971_m1), TLR8 (Hs00152972_m1) e TLR9 (Hs00152973_m1).

Techniques: Expressing, Control

Journal: Einstein

Article Title: Regulatory T cells isolated from endometriotic peritoneal fluid express a different number of Toll-like receptors

doi: 10.31744/einstein_journal/2020AO5294

Figure Lengend Snippet: Expressão de receptores do tipo Toll por linfócitos T reguladores peritoneais de pacientes com endometriose e controles

Article Snippet: Foram usados testes TaqMan de expressão gênica (Applied Biosystems) para os genes-alvo TLR1 (Hs00413978_m1), TLR2 (Hs00610101_m1), TLR3 (Hs01551078_m1), TLR4 (Hs00152939_m1), TLR5 (Hs00152825_m1), TLR6 (Hs00271977_s1), TLR7 (Hs00152971_m1), TLR8 (Hs00152972_m1) e TLR9 (Hs00152973_m1).

Techniques:

Journal: Biochimica et biophysica acta

Article Title: Toll-like receptor 5 deficiency attenuates interstitial cardiac fibrosis and dysfunction induced by pressure overload by inhibiting inflammation and the endothelial-mesenchymal transition.

doi: 10.1016/j.bbadis.2015.08.013

Figure Lengend Snippet: Figure 1 TLR5 expression in the myocardium after AB-induced pressure overload. Western

Article Snippet: Before TGF-β1 treatment, the HUVEC-12 cells were transfected with a plasmid over-expressing TLR5 (Ad-TLR5, Addgene, 13019) using FuGENE®HD Transfection Reagent (Promega, E2311).

Techniques: Expressing, Western Blot

Journal: Biochimica et biophysica acta

Article Title: Toll-like receptor 5 deficiency attenuates interstitial cardiac fibrosis and dysfunction induced by pressure overload by inhibiting inflammation and the endothelial-mesenchymal transition.

doi: 10.1016/j.bbadis.2015.08.013

Figure Lengend Snippet: Figure 2 the expression of TLR5 in cultured neonatal rat cardiomyocytes, H9c2

Article Snippet: Before TGF-β1 treatment, the HUVEC-12 cells were transfected with a plasmid over-expressing TLR5 (Ad-TLR5, Addgene, 13019) using FuGENE®HD Transfection Reagent (Promega, E2311).

Techniques: Expressing, Cell Culture

Journal: Biochimica et biophysica acta

Article Title: Toll-like receptor 5 deficiency attenuates interstitial cardiac fibrosis and dysfunction induced by pressure overload by inhibiting inflammation and the endothelial-mesenchymal transition.

doi: 10.1016/j.bbadis.2015.08.013

Figure Lengend Snippet: Figure 3 The effects of TLR5 on cardiac dysfunction. Statistical analysis of the HW/BW ratio,

Article Snippet: Before TGF-β1 treatment, the HUVEC-12 cells were transfected with a plasmid over-expressing TLR5 (Ad-TLR5, Addgene, 13019) using FuGENE®HD Transfection Reagent (Promega, E2311).

Techniques:

Journal: Biochimica et biophysica acta

Article Title: Toll-like receptor 5 deficiency attenuates interstitial cardiac fibrosis and dysfunction induced by pressure overload by inhibiting inflammation and the endothelial-mesenchymal transition.

doi: 10.1016/j.bbadis.2015.08.013

Figure Lengend Snippet: Figure 4 TLR5 deficiency attenuates the fibrotic response induced by pressure overload.

Article Snippet: Before TGF-β1 treatment, the HUVEC-12 cells were transfected with a plasmid over-expressing TLR5 (Ad-TLR5, Addgene, 13019) using FuGENE®HD Transfection Reagent (Promega, E2311).

Techniques:

Journal: Scientific Reports

Article Title: Diet influences the functions of the human intestinal microbiome

doi: 10.1038/s41598-020-61192-y

Figure Lengend Snippet: The intestinal microbiota induces the expression of interleukins, Toll-like receptor 5 and the lectin RegIIIα. The capacity of microbial fecal cells (MFCs) extracted from 22 omnivores (O), 20 vegetarians (VG) and 19 vegans (V) and the corresponding microbial protein cell extracts (MPCEs) and flagellin (FlC) at two different concentrations (0.015 µg/ml of DMEM for O and 0.090 µg/ml for VG and V MPCE samples) to affect the expression of IL-8 (panels A–C), IL-22 (D–F) and IL-23 (G–I), Toll-like receptor 5 (J–L) and the lectin RegIIIα (M–O) was assessed at 6 and 24 h in human HT29 colon cancer cells. The levels of expression of each gene in colon cancer cells treated with MFCs, MPCEs or FlC, compared to control (cells treated with PBS only), presented as the mean value ± s.d. from three replicates for each of the 61 samples. All data shown are representative of three independent experiments using fecal samples collected at three time-points in one month. Differences between control and treated cells were considered statistically significant when p < 0.05. A schematic representation of how interleukins, TLR-5 and RegIIIα work synergically is provided in panel P.

Article Snippet: The cDNA was amplified and detected through Taqman primer-probe sets (Applied Biosystems) (IL8, Hs00174103_m1; IL22, Hs01574154_m1; IL23A, Hs00372324_m1; TLR5, Hs01920773_s1; and REG3A, Hs00170171_m1).

Techniques: Expressing, Control

Journal: Cell reports

Article Title: Microbial Exposure Enhances Immunity to Pathogens Recognized by TLR2 but Increases Susceptibility to Cytokine Storm through TLR4 Sensitization

doi: 10.1016/j.celrep.2019.07.028

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: TaqManGene Expression Assay (FAM) - Assay ID: Mm00546288_s1; Gene Symbol: Tlr5 , Life Technologies , Cat #: 4453320.

Techniques: Virus, Recombinant, Sequencing, Expressing, Software